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Morphometric analysis of experimentally induced periapical lesions: radiographic vs histopathological findings

¹ Department of Pathology, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil; ² Department of Pediatric Dentistry, School of Dentistry of Piracicaba, State University of Campinas, Piracicaba, São Paulo, Brazil; ³ Department of Pediatric Dentistry, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

*Correspondence to: Andiara De Rossi, Department of Pathology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14049-900 Ribeirão Preto, São Paulo, Brazil; Email: andiaraderossi{at}bol.com.br

Received 18 January 2006; revised 20 April 2006; accepted 23 May 2006

Objectives: To evaluate the accuracy and reliability of conventional (Kodak Ektaspeed Plus^TM film) and digitized radiographic images to detect the presence as well to estimate the size, as measured by an image analysis programme, of periapical radiolucencies induced in dog teeth in comparison with the histomorphometric data obtained from the same lesions by conventional and fluorescence microscopy.

Method: After the removal of pulp, the root canals of five premolars from the same animal were left exposed for 7 days after which they were sealed for 60 days. At day 53, three more premolars were opened and left exposed to the oral cavity for 7 days. Intact premolars were used as control. Conventional radiographs were taken at day 0, day 7, day 30, day 45 and day 60. Morphometry in digitized radiographic images and histological sections were compared at day 7 and day 60 after setting the experimental series.

Results: Radiographically, periapical lesions were only detected 30 days after coronal sealing. A progressively increasing radiolucent lesion area was observed at day 45 and day 60. Histopathologically, 7 days after pulp removal dense inflammatory infiltrate and root resorption in the periapical region was observed. At day 7 and day 60, the lesion sizes were similar when evaluated by both conventional and fluorescence microscopy. Lesion size was about 20% larger in digitized radiographs in comparison with histological measurements.

Conclusions: Although image digitization could not improve the detection of the early stages of periapical lesions, it provides a valuable quantitative assessment of extensive periapical lesions. In addition, fluorescence light microscopy enhances the visualization of the apical and periapical structures and seems to be a highly useful tool for histological evaluation, valuable for both qualitative and quantitative studies of periapical disease.

Keywords: radiography; microscopy; fluorescence; periapical disease